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Microscopy
FLOW CYTOMETRY

Leica scope

The Leica DMI6000 fluorescent microscope can be used for a variety of applications in fluorescence microscopy and image analysis, including time lapse experiments. It has YFP, CFP, GFP, Texas Red, Cy5, A4 filter cubes. It is supplied with the LAS and LAS AF soltware. Objectives include: Dry (2.5x, 10x, 20x and 40x) and Oil (40x and 60x/100x). The Leica DMI6000 has a colour camera in addition to the fluorescence camera and can be used for bright field, DIC, phase contrast as well. Individuals who wish to use the Leica scope must contact Dr. Elizabeth Fidalgo for more information and training. Click here for Fees and Biosafety details.

 

  • User fee – $ 25.00/hour – annual charge for the total of hours used from April to March.
  • Cap fee – $ 1000.00 for 40 to 100 hrs and $ 2000.00 for more than 100 hrs per year.
  • Overnight timelapse imaging – $ 75.00 for an experiment.
  • Training fee – $ 25.00 per person – Training session is done during the first week of each month.

  • The room CORe-104 is BSL2. Please, check the SOP binder located in the room before using the scope.
  • No radiolabeled material is permitted.
  • No food or drink is permitted inside the room.
  • No gloves should be worn when operating the scopes.

Confocal scope

e confocal microscope, a Biorad 1024 on a Nikon Eclipse 800 microscope, has the capability of combining double label imaging with DIC transmission optics. It has a krypton/argon laser with excitation at 488 nm, 568 nm and 647 nm. Objectives include 20x, 40x oil and 60x oil. The software includes quantitative analysis for distance,staining intensity and double-label co-localization. Triple label sequential analysis is also available. A cryostat (Micron) is available for preparing sections for confocal imaging. Individuals who require confocal image analysis in their research advised to contact Dr. Andrew Swan for more information

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BD LSR Fortessa™ X‐20

  • 488 nm (Blue)
  • 640 nm (Red)
  • 405 nm (Violet)
  • 561 nm (Yellow/Green)
  • 355 nm (UV)

  • Blue ‐ FITC (530/30BP, 505LP), PerCP Cy5.5 (695/40BP, 685LP)
  • Red ‐ APC (660/20BP), AF700 (730/45BP, 695LP), APC‐Cy7/APC‐H7 (780/60BP, 750LP)
  • YG – PE/DsRed (582/12BP), PE‐TR/mCherry (610/20BP, 550LP), PE‐Cy5 (670/30BP, 635LP), PE‐Cy5.5 (710/50BP, 695LP), PE‐Cy7 (780/60BP, 750LP)
  • Violet ‐ BV421/Pac Blue (450/50BP), BV510/AmCyan (528/50BP, 475LP), BV605 (610/20, 595LP), BV650 (670/30BP, 635LP), BV711 (710/50BP, 695LP), BV786 (780/60BP,750LP)
  • UV – Indo‐1 (violet)/AF350/DAPI/Hoechst (450/50BP), Hoechst Red/Side Population (675LP)

BD FACSAria Fusion™

  • 488 nm (Blue)
  • 640 nm (Red)
  • 405 nm (Violet)
  • 561 nm (Yellow/Green)
  • 355 nm (UV)

  • Blue – FITC (530/30BP 502LP), PerCP‐Cy5.5 (695/40BP 655LP)
  • Red – APC (660/20BP), APC‐Cy7 (780/60BP 735LP), AF 700(760/45BP, 690LP)
  • Violet – BV421/Pac Blue (450/40), BV510/AmCyan (510/50BP, 502LP), BV 605 (610/20BP, 600LP), BV650 (660/20BP, 630LP), BV650 (670/30BP, 635LP), BV711 (710/50BP,
    695LP)
  • PE/DsRed (582/15BP), PE‐TR/mCherry, PI (610/20BP 600LP), PE‐Cy5 (670/30BP, 635LP), PE‐Cy5.5 (710/50BP 630LP), PE‐Cy7 (780/60BP 735LP)
  • UV – Indo‐1 (violet)/AF350/DAPI/Hoechst (450/50BP), BUV395 (378/28BP)

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