FLOW CYTOMETRY CORE BIOSAFETY

The University of Windsor Flow Core Facility has BSL2 permit, no BSL3 samples are allowed in the Facility.

 

    • All requests for analyzing and sorting samples must be accompanied by the Biosafety
      form, sign by the principal investigator (PI)
    • BSL2 samples must be fixed before they are analyzed on the cytometer, these samplesinclude: All infectious, human, and non-human primate cells, cells manipulated with viralagents (EBV, vaccinia, HTLV, lentivirus, nanoparticle, etc). If the in vivo model is

      considered BSL2 then your sample is a BSL2 sample.

    • Primary human samples used for sorting need to have the screening results and benegative for: Hepatitis B, C, HIV, Mycobacterium tuberculosis, Neisseria meningitides,Chlamydia psittacci, Coxiella burnetii, HTLV-1,2, LVMV, vesicular stomatitis virus

      infections.

    • Samples manipulated with viral agents: EBV, vaccinia, HTLV, lentivirus, nanoparticle,etc must be clearly described in the Biosafety form. These samples need to be washed atleast 3 times and passed through a 70 micron nylon filter just prior to being brought to this

      facility.

    • Gloves should be worn by all users at all times.
    • No food or drink is permitted.
    • During sign-up procedure users must notify the flow facility manager about the biosafetylevel of the material to be analyzed or sorted.
    • No radiolabeled samples are permitted on any instrument.
    • All biological waste must be removed from the facility for disposal in their homelaboratory.