FLOW CYTOMETRY CORE FACILITY


x20

BD LSR Fortessa™ X‐20 :5‐laser 18‐Colour (2B/3R/5YG/6V/2UV)

  • 488 nm (Blue)
  • 640 nm (Red)
  • 405 nm (Violet)
  • 561 nm (Yellow/Green)
  • 355 nm (UV)

Standard Configuration

  1. Blue ‐ FITC (530/30BP, 505LP), PerCP Cy5.5 (695/40BP, 685LP)
  2. Red ‐ APC (660/20BP), AF700 (730/45BP, 695LP), APC‐Cy7/APC‐H7 (780/60BP, 750LP)
  3. YG – PE/DsRed (582/12BP), PE‐TR/mCherry (610/20BP, 550LP), PE‐Cy5 (670/30BP, 635LP), PE‐Cy5.5 (710/50BP, 695LP), PE‐Cy7 (780/60BP, 750LP)
  4. Violet ‐ BV421/Pac Blue (450/50BP), BV510/AmCyan (528/50BP, 475LP), BV605 (610/20, 595LP), BV650 (670/30BP, 635LP), BV711 (710/50BP, 695LP), BV786 (780/60BP,750LP)
  5. UV – Indo‐1 (violet)/AF350/DAPI/Hoechst (450/50BP), Hoechst Red/Side Population (675LP)

fusion

BD FACSAria Fusion™ 5‐Laser 18‐Colour, with ACDU (2B/3R/6V/5YG/2UV)

  • 488 nm (Blue)
  • 640 nm (Red)
  • 405 nm (Violet)
  • 561 nm (Yellow/Green)
  • 355 nm (UV)

Standard Configuration

  1. Blue – FITC (530/30BP 502LP), PerCP‐Cy5.5 (695/40BP 655LP)
  2. Red – APC (660/20BP), APC‐Cy7 (780/60BP 735LP), AF 700(760/45BP, 690LP)
  3. Violet – BV421/Pac Blue (450/40), BV510/AmCyan (510/50BP, 502LP), BV 605 (610/20BP, 600LP), BV650 (660/20BP, 630LP), BV650 (670/30BP, 635LP), BV711 (710/50BP,
    695LP)
  4. PE/DsRed (582/15BP), PE‐TR/mCherry, PI (610/20BP 600LP), PE‐Cy5 (670/30BP, 635LP), PE‐Cy5.5 (710/50BP 630LP), PE‐Cy7 (780/60BP 735LP)
  5. UV – Indo‐1 (violet)/AF350/DAPI/Hoechst (450/50BP), BUV395 (378/28BP)

REQUEST TO HAVE SAMPLE ANALYZED OR SORTED

Analyzer – X20
The request should be done at least one week before of the possible date for the analyze, the forms below should be submitted with the request:

  • Analyzer sample form
  • Biosafety form
Sorter – Fusion
The request should be done at least 2 weeks before the possible date for the sorter, the forms below should be submitted with the request:

  • Sorter sample form
  • Biosafety form